By Barbara Vida on Tuesday, 23 March 2021
Category: SPECT/CT

Early detection of colorectal cancer

SPECT imaging evaluation of 111indium-chelated cetuximab for diagnosing EGFR-positive tumor in an HCT-15-induced colorectal xenograft

Bin-Bin Shiha, Yi-Fang Changb, Chun-Chia Chengb, Hao-Jhih Yangc, Kang-Wei Changc, Ai-Sheng Hoa, Hua-Ching Lind, Chun Yeha, Chun-Chao Change,f

a Division of Gastroenterology, Cheng Hsin General Hospital, Taipei, Taiwan, ROC

b Hematology and Oncology, Mackay Memorial Hospital, Taipei, Taiwan, ROC

c Institute of Nuclear Energy Research, Atomic Energy Council, Taoyuan, Taiwan, ROC

d Division of Proctology, Cheng Hsin General Hospital, Taipei, Taiwan, ROC

e Division of Gastroenterology and Hepatology, Department of Internal Medicine, Taipei Medical University Hospital, Taipei, Taiwan, ROC

f Division of Gastroenterology and Hepatology, Department of Internal Medicine, School of Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan, ROC

http://dx.doi.org/10.1016/j.jcma.2017.02.010

Summary

Colorectal cancer (CRC) occurs with high incidence worldwide, but is usually diagnosed in late stage with metastasis by the conventional methods. Epidermal growth factor receptor (EGFR) is overexpressed in 97% of CRC cells, serving a promising diagnostic candidate. In the present study, Cetuximab, an anti-EGFR monoclonal antibody was conjugated with an isotope chelator, diethylene triamine penta acetic acid (DTPA), labeled with 111indium (111In) and injected to tumor bearing mice. Biological distrubution was investigated by SPECT/CT imaging.

Results revealed that 111In-Cetuximab accumulated in the both small (50mm3) and large (250mm3) tumors, whereas the ratio of tumor to muscle in the large tumor was 7.5-fold. The biodistribution data indicated that the 111In-cetuximab bound to tumor specifically that was higher than that in other organs. Consequently, 111In-cetuximab is suggested to be suitable for early diagnosis and prognostic monitor of EGFR-positive CRC in further clinical practice.

Results from nanoSPECT/CT

 

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